Molecular detection of Mycoplasma pneumoniae in respiratory samples from hospitalized children
DOI:
https://doi.org/10.31053/1853.0605.v80.n1.37514Keywords:
mycoplasma pneumoniae, pneumonia, respiratory tract infectionsAbstract
It is currently reported that Mycoplasma pneumoniae (MP) infection can occur at a young age.
Objective: Describe the findings of the molecular detection of MP in respiratory secretions of patients who required hospitalization due to acute respiratory infection, in a pediatric hospital.
Population and methods: Data collection was performed by reviewing medical records and statistical correlation using the chi-square test. 919 patients from one month to fourteen years and eleven months of age who required hospitalization for acute respiratory infection were included. The frequency by age and sex of the isolation of MP was analyzed together with other respiratory pathogens.
Results: MP was the most frequently detected microorganism (30%), followed by Respiratory Syncytial Virus (RSV) (25.1%). Age and sex did not behave as a predictor of detection for MP. In 47.3% of the patients, PM was isolated together with another pathogen, the most prevalent was RSV 31.3%. Regarding the diagnoses at discharge of the group of patients with isolation of MP and a another microorganism, 50.8% had bronchiolitis, and in the group of patients with identification only of PM this percentage was 32.4%. The difference in the distributions was statistically significant (p <0.05).
Conclusion: We conclude that MP detection is frequent in our environment and occurs in a significant number of cases together with another respiratory pathogen. These findings should prompt further study to determine their clinical relevance.
Downloads
References
1- Argentina. Ministerio de Salud. Boletín Integrado de Vigilancia. 2019(479)SE 52. [Acceso: el 29 de diciembre de 2020]. Disponible en: https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&ved=2ahUKEwjA6qPe7e39AhUYqZUCHcBoAUcQFnoECAgQAQ&url=https%3A%2F%2Fbancos.salud.gob.ar%2Fsites%2Fdefault%2Ffiles%2F2020-01%2Fboletin-integrado-vigilancia-n479.pdf&usg=AOvVaw3_GavHQG6NfEE3Qs1bONam
2- Marcone DN, Ellis A, Videla C, Ekstrom J, Ricarte C, Carballal G, Vidaurreta SM, Echavarría M. Viral etiology of acute respiratory infections in hospitalized and outpatient children in Buenos Aires, Argentina. Pediatr Infect Dis J. 2013 Mar;32(3):e105-10. doi: 10.1097/INF.0b013e31827cd06f.
3- Arnold FW, Summersgill JT, Lajoie AS, Peyrani P, Marrie TJ, Rossi P, Blasi F, Fernandez P, File TM Jr, Rello J, Menendez R, Marzoratti L, Luna CM, Ramirez JA; Community-Acquired Pneumonia Organization (CAPO) Investigators. A worldwide perspective of atypical pathogens in community-acquired pneumonia. Am J Respir Crit Care Med. 2007 May 15;175(10):1086-93. doi: 10.1164/rccm.200603-350OC.
4- Othman N, Isaacs D, Kesson A. Mycoplasma pneumoniae infections in Australian children. J Paediatr Child Health. 2005 Dec;41(12):671-6. doi: 10.1111/j.1440-1754.2005.00757.x.
5- Bosnak M, Dikici B, Bosnak V, Dogru O, Ozkan I, Ceylan A, Haspolat K. Prevalence of Mycoplasma pneumoniae in children in Diyarbakir, the south-east of Turkey. Pediatr Int. 2002 Oct;44(5):510-2. doi: 10.1046/j.1442-200x.2002.01606.x.
6- Ferrero FC, Ossorio MF. Eriksson PV, Durán AP. Mycoplasma pneumoniae en niños con neumonía. Arch Argent Pediatr. 2000; 98(1):12-17
7- Inostroza E, Pinto R. Neumonía por agentes atípicos en niños. Revista Médica Clínica Las Condes. 2017;28(1): 90-96. doi: 10.1016/j.rmclc.2017.01.006
8- Atkinson TP, Waites KB. Mycoplasma pneumoniae Infections in Childhood. Pediatr Infect Dis J. 2014 Jan;33(1):92-4. doi: 10.1097/INF.0000000000000171.
9- Defilippi A, Silvestri M, Tacchella A, Giacchino R, Melioli G, Di Marco E, Cirillo C, Di Pietro P, Rossi GA. Epidemiology and clinical features of Mycoplasma pneumoniae infection in children. Respir Med. 2008 Dec;102(12):1762-8. doi: 10.1016/j.rmed.2008.06.022.
10- Chang HY, Chang LY, Shao PL, Lee PI, Chen JM, Lee CY, Lu CY, Huang LM. Comparison of real-time polymerase chain reaction and serological tests for the confirmation of Mycoplasma pneumoniae infection in children with clinical diagnosis of atypical pneumonia. J Microbiol Immunol Infect. 2014 Apr;47(2):137-44. doi: 10.1016/j.jmii.2013.03.015.
11- Gordon O, Oster Y, Michael-Gayego A, Marans RS, Averbuch D, Engelhard D, Moses AE, Nir-Paz R. The Clinical Presentation of Pediatric Mycoplasma pneumoniae Infections-A Single Center Cohort. Pediatr Infect Dis J. 2019 Jul;38(7):698-705. doi: 10.1097/INF.0000000000002291.
12- Lu Y, Wang Y, Hao C, Ji W, Chen Z, Jiang W, Yan Y, Gu W. Clinical characteristics of pneumonia caused by Mycoplasma pneumoniae in children of different ages. Int J Clin Exp Pathol. 2018 Feb 1;11(2):855-861.
13- Søndergaard MJ, Friis MB, Hansen DS, Jørgensen IM. Clinical manifestations in infants and children with Mycoplasma pneumoniae infection. PLoS One. 2018 Apr 26;13(4):e0195288. doi: 10.1371/journal.pone.0195288.
14- Aguilera-Alonso D, López Ruiz R, Centeno Rubiano J, Morell García M, Valero García I, Ocete Mochón MD, Montesinos Sanchis E. Epidemiological and clinical analysis of community-acquired Mycoplasma pneumonia in children from a Spanish population, 2010-2015. An Pediatr (Engl Ed). 2019 Jul;91(1):21-29. doi: 10.1016/j.anpede.2019.01.003.
15- Del Valle-Mendoza J, Orellana-Peralta F, Marcelo-Rodríguez A, Verne E, Esquivel-Vizcarra M, Silva-Caso W, Aguilar-Luis MA, Weilg P, Casabona-Oré V, Ugarte C, Del Valle LJ. High Prevalence of Mycoplasma pneumoniae and Chlamydia pneumoniae in Children with Acute Respiratory Infections from Lima, Peru. PLoS One. 2017 Jan 27;12(1):e0170787. doi: 10.1371/journal.pone.0170787.
16- Sanluis Fenelli G, Chiolo MJ, Torres FA, Balbaryski J, Domínguez P, Ossorio MF, Rial MJ, Ferrero F. Seroprevalencia de anticuerpos anti-Mycoplasma pneumoniae en niños menores de 12 años [Seroprevalence of anti-Mycoplasma pneumoniae antibodies in otherwise healthy children]. Rev Chil Pediatr. 2020 Jun;91(3):347-352. Spanish. doi: 10.32641/rchped.v91i3.1476.
17- Lezcano A, Balbaryski J, Torres F, Cutri A, Coarasa A, Ossorio MF, Ferrero F. Seroprevalencia de anticuerpos anti-Mycoplasma pneumoniae: evaluación en niños menores de 12 años [Seroprevalence of Mycoplasma pneumoniae in children aged under 12 years]. Arch Argent Pediatr. 2008 Feb;106(1):6-10. Spanish.
18- Chiu CY, Chen CJ, Wong KS, Tsai MH, Chiu CH, Huang YC. Impact of bacterial and viral coinfection on mycoplasmal pneumonia in childhood community-acquired pneumonia. J Microbiol Immunol Infect. 2015 Feb;48(1):51-6. doi: 10.1016/j.jmii.2013.06.006.
19- Gentile A, Lucion MF, Juarez MDV, Areso MS, Bakir J, Viegas M, Mistchenko A. Burden of Respiratory Syncytial Virus Disease and Mortality Risk Factors in Argentina: 18 Years of Active Surveillance in a Children's Hospital. Pediatr Infect Dis J. 2019 Jun;38(6):589-594. doi: 10.1097/INF.0000000000002271.
20- Gil J, Almeida S, Constant C, Pinto S, Barreto R, Melo Cristino J, Machado MDC, Bandeira T. Short-term relevance of lower respiratory viral coinfection in inpatients under 2 years of age. An Pediatr (Engl Ed). 2018 Mar;88(3):127-135. doi: 10.1016/j.anpede.2017.03.006.
21- Chen LL, Cheng YG, Chen ZM, Li SX, Li XJ, Wang YS. [Mixed infections in children with Mycoplasma pneumoniae pneumonia]. Zhonghua Er Ke Za Zhi. 2012 Mar;50(3):211-5. Chinese.
22- Song Q, Xu BP, Shen KL. Effects of bacterial and viral co-infections of mycoplasma pneumoniae pneumonia in children: analysis report from Beijing Children's Hospital
between 2010 and 2014. Int J Clin Exp Med. 2015 Sep 15;8(9):15666-7.
23- Kim JH, Kim EJ, Kwon JH, Seo WH, Yoo Y, Choung JT, Song DJ. Clinical characteristics of respiratory viral coinfection in pediatric Mycoplasma pneumoniae pneumonia. Allergy Asthma Respir Dis 2017;5:15–20.
24- Qiu H, Wu J, Hong L, Luo Y, Song Q, Chen D. Clinical and epidemiological features of 36 children with coronavirus disease 2019 (COVID-19) in Zhejiang, China: an observational cohort study. Lancet Infect Dis. 2020 Jun;20(6):689-696. doi: 10.1016/S1473-3099(20)30198-5
25- Hoang A, Chorath K, Moreira A, Evans M, Burmeister-Morton F, Burmeister F, Naqvi R, Petershack M, Moreira A. COVID-19 in 7780 pediatric patients: A systematic review. EClinicalMedicine. 2020 Jun 26;24:100433. doi: 10.1016/j.eclinm.2020.100433
26- Moynihan KM, Barlow A, Nourse C, Heney C, Schlebusch S, Schlapbach LJ. Severe Mycoplasma Pneumoniae Infection in Children Admitted to Pediatric Intensive Care. Pediatr Infect Dis J. 2018 Dec;37(12):e336-e338. doi: 10.1097/INF.0000000000002029.
27- Kutty PK, Jain S, Taylor TH, Bramley AM, Diaz MH, Ampofo K, Arnold SR, Williams DJ, Edwards KM, McCullers JA, Pavia AT, Winchell JM, Schrag SJ, Hicks LA. Mycoplasma pneumoniae Among Children Hospitalized With Community-acquired Pneumonia. Clin Infect Dis. 2019 Jan 1;68(1):5-12. doi: 10.1093/cid/ciy419
28- Gao J, Xu L, Xu B, Xie Z, Shen K. Human adenovirus Coinfection aggravates the severity of Mycoplasma pneumoniae pneumonia in children. BMC Infect Dis. 2020 Jun 16;20(1):420. doi: 10.1186/s12879-020-05152-x.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Universidad Nacional de Córdoba
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The generation of derivative works is allowed as long as it is not done for commercial purposes. The original work may not be used for commercial purposes.
How to Cite
