Métodos cromatográficos optimizados para la identificación y cuantificación de terpenos en aceite de Cannabis sativa de uso medicinal

Leonardo Bajda; María Marcela Amaro; Guillermina A. Bongiovanni

doi:10.31053/1853.0605.v80.n2.39593

Authors

Leonardo Bajda PROBIEN (Institute for Research and Development in Process Engineering, Biotechnology and Alternative Energies), CONICET - National University of Comahue, CCT-Patagonia Confluencia.
María Marcela Amaro PROBIEN (Institute for Research and Development in Process Engineering, Biotechnology and Alternative Energies), CONICET - National University of Comahue, CCT-Patagonia Confluencia.
PhD PROBIEN (Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas, CONICET-Universidad Nacional del Comahue

DOI:

https://doi.org/10.31053/1853.0605.v80.n2.39593

Keywords:

terpenes, gas chromatography, quality control, Cannabis sativa

Abstract

Introduction: Cannabis sativa is a plant species with numerous active principles, so the list of its therapeutic uses is expanding. In this sense, there are numerous evidences of the possible medicinal use of terpenes, as well as their synergism with cannabinoids (entourage effect). Thus, as more countries contemplate the legalization and authorization of medical cannabis, the number of cannabis extraction and analysis laboratories is increasing to meet the demand, requiring adequate analytical tools. Methodology: In response to numerous inquiries from physicians, analytical laboratories and users, the PROBIEN chromatography laboratory has selected two methods for the analysis of terpenes in Cannabis oil by gas chromatography technique (GC-FID). The methods are described using HP-5 and Innowax columns. The external standard method was used for the quantitative determination of β-Pinene, Myrcene, p-Cymene, Limonene, Linalool, α-Terpineol, Nerol and Geraniol. Results: good peak separation and reproducibility were observed, appropriate for the identification and quantification of the main terpenes in Cannabis extracts. The area/concentration ratio was linear in the range of 0.0005 to 2.0 mg/ml. Main conclusion: The described methods allow the identification and quantification of the major terpenes in Cannabis oil for an adequate quality control.

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Author Biographies

Leonardo Bajda, PROBIEN (Institute for Research and Development in Process Engineering, Biotechnology and Alternative Energies), CONICET - National University of Comahue, CCT-Patagonia Confluencia.

Member of the CONICET Research Support Staff Career.

Responsible for the Chromatography Laboratory of PROBIEN since 2016, for the implementation of chromatographic analytical methods and the analysis of different samples for internal or external researchers

Responsible for several High Level Technical Services provided at PROBIEN to internal and external users for GC-FID and HPLC-DAD analysis.
María Marcela Amaro, PROBIEN (Institute for Research and Development in Process Engineering, Biotechnology and Alternative Energies), CONICET - National University of Comahue, CCT-Patagonia Confluencia.

Member of the CONICET Research Support Staff Career.

Co-responsible for the Chromatography Laboratory of PROBIEN since 2017, for the implementation of chromatographic analytical methods and analysis of different samples for internal or external researchers or applicants. With previous experience in different analytical methods at the National University of La Plata.
PhD, PROBIEN (Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas, CONICET-Universidad Nacional del Comahue

PhD. in Chemical Sciences Guillermina A. Bongiovanni

Independent Researcher of CONICET and Professor of General Agricultural Chemistry. Director of the Environmental Biotechnology Group of PROBIEN.

guillermina.bongiovanni@probien.gob.ar

https://orcid.org/0000-0001-7877-2586

PROBIEN (Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas, CONICET-Universidad Nacional del Comahue, Buenos Aires 1400, Neuquén Capital (CP 8300)

Faculty of Agricultural Sciences, Universidad Nacional del Comahue, RP 151, km 12, Cinco Salto (Río Negro).

The three authors are responsible for the High Level Technical Service (STAN ST4856 "Quantitative analysis of cannabinoids and terpenes in oils, tinctures and extracts of cannabis") provided at PROBIEN. This and other services provided by the Chromatography Laboratory of PROBIEN, are the main source of funding to maintain the equipment, purchase supplies and implement the methodologies of analysis by GC-FID and HPLC-DAD.

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