Exudados radicales de alfalfa (Medicago sativa L.) en condiciones de estrés salino. Expresión de genes nod en Rhizobium
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El estrés salino puede interferir con la transcripción de los genes de nodulación (nod) en Rhizobium al modificar la composición de los exudados radicales o la respuesta de las rizobias. Se recolectaron exudados de raíces de alfalfa (Medicago sativa L.) crecidas en NaCl al 0,5% durante 6 días y se analizaron mediante cromatografía en capa fina (TLC). La inducción de la transcripción de los genes nod de rizobias se llevó a cabo utilizando un vector de expresión con la fusión nod-lacZ. Los resultados indican que los perfiles de exudados de las plantas tratadas difieren en el tiempo con respecto a los controles, y que este retardo inducido por la concentración salina no afecta la capacidad inductora de los genes nod.
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