Radical exudates of alfalfa (Medicago sativa L.) under salt stress conditions. Expression of nod genes in Rhizobium
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Abstract
A stress condition such as salinity may interfere with the transcription of nodulation (nod) genes in Rhizobium by modifying the composition of root exudates or the response of rhizobia. Root exudates of alfalfa (Medicago sativa L.) grown in 0.5% NaCl were collected over 6 days and analyzed by thin-layer chromatography (TLC). The induction of transcription of rhizobia nod genes was performed using an expression vector with the nod-lacZ fusion. The results indicate that the exudate patterns of treated plants differ from the controls over time, and that this delay induced by salt concentration does not affect the induction capacity of nod genes.
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