Micropropagation of Actinidia chinensis Planch (Kiwi). Optimization of the culture medium for large-scale clonal propagation
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Abstract
Micropropagation of Actinidia chinensis Planch. cv. Hayward (kiwi) plants was possible by the culture of resting buds excised in August. The initiation of explant growth and the proliferation of axillary buds were achieved using the MS (1962) salt formulation, organic compounds from Standardi (1983), 20 g/L sucrose, and 1 mg/L BA and 0.1 mg/L GA3. After 30 days, a multiplication rate of 4-5 was obtained. Root formation was possible when the macronutrient composition of MS was reduced by half, with the same sucrose concentration and the addition of IBA and PPZ, both at 1 mg/L. A rooting percentage of 95% was obtained after 30 days. The presented protocol resulted in the production of nearly 1000 hardened plantlets.
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