Evaluation of the in vitro dedifferentiation and redifferentiation capacity of four genotypes of Lycopersicon esculentum Mill
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Abstract
Four Lycopersicon esculentum genotypes—Planeuco INTA, Rossol Selección La Consulta, Cal J, and Raci 53—were evaluated to produce callus and further regeneration. Folioli were used as explants. The basal medium for callus production was MS with different concentrations of plant regulators: ANA 2 mg·L⁻¹ + BAP 1 mg·L⁻¹ (MI) and ANA 1 mg·L⁻¹ + BAP 0.5 mg·L⁻¹ (MII). The genotypes were analyzed under two environmental conditions: light and darkness. Regeneration media was the same but with different concentrations of plant regulators: AIA 0.175 mg·L⁻¹ + BAP 2.25 mg·L⁻¹ (MA) and AIA 0.175 mg·L⁻¹ + BAP 4.50 mg·L⁻¹ (MB). Callus percentage was evaluated after 30 days from in vitro culture. The percentage of redifferentiation was low and depended on the previous conditions they were exposed to.
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