Determination of Verticillium dahliae Kleb. pathotypes from the olive regions of central and western Argentina and virulence tests in cotton
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Abstract
Verticillium wilt caused by the soil-borne fungus Verticillium dahliae Kleb. is a disease that affects olive (Olea europaea L.) and cotton crops, among others. According to their pathogenicity, V. dahliae isolates can be classified as defoliating (D) and non-defoliating (ND). The objectives of this study were: i) to determine the pathotypes of a collection of 42 V. dahliae isolates from olive plants in the provinces of Catamarca, Córdoba, La Rioja and Mendoza kept at IPAVE-INTA Córdoba, and (ii) to evaluate the virulence of eight recently isolated isolates belonging to this collection. Nested PCR was used for molecular analysis of the 42 fungal isolates and virulence evaluation was performed by artificial inoculations on cotton seedlings with a completely randomized design. The total of the isolates was characterized as ND. Two strains from La Rioja, two from Catamarca and one from Mendoza, were significantly more virulent (p< 0.05) for the relative area under disease progress curve, final mean severity, average severity percentage and severity index.
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Al Taae, H. (2020). Using nested PCR to detect the nondefoliating pathotype of Verticillium dahliae on olive orchard. Crop Research, 54 (5 y 6), 139-142. DOI: http://dx.doi.org/10.31830/2454-1761.2019.023
Baídez, A. G., Gómez, P., del Río, J. A. y Ortuño, A. (2007). Dysfunctionality of the xylem in Olea europaea L. plants associated with the infection process by Verticillium dahliae Kleb. Role of phenolic compounds in plant defense mechanisms. Journal of Agricultural and Food Chemistry, 55 (9), 3373-3377. DOI: https://doi.org/10.1021/jf063166d
Baytar, A. A., Erdogan, O., Frary, A., Frary, A. y Doganlar, S. (2017). Molecular diversity and identification of alleles for Verticillium wilt resistance in elite cotton (Gossypium hirsutum L.) germplasm. Euphytica, 213, 31. DOI: https://doi.org/10.1007/s10681-016-1787-y
Bejarano Alcázar, J., Blanco López, M. A., Melero Vara, J. M. y Jiménez Diaz, R. M. (1996). Etiology, importance, and distribution of Verticillium wilt of cotton in southern
Spain. Plant Disease, 80 (11), 1233–1238.
Blanco López, M. A., Rodríguez Jurado, D. y Jiménez Díaz, R. M. (1994). La Verticilosis del Olivo. Agricultura, 746, 777 – 780.
Bonacic, I., Ojeda, A. D., Casse, M. F., Garay, O., Coronel, J. y Garay, J. (2012). Ensayos de campo con inoculación, en diferentes momentos del ciclo de cultivo con Verticillium dahliae para evaluar pérdidas de rendimiento. En: Memoria técnica Área de investigación en Mejoramiento genético y Protección Vegetal. Chaco, Argentina: Instituto Nacional de Tecnología Agropecuaria Estación Experimental Agropecuaria Sáenz Peña.
Campbell, C. L. y Madden, L. V. (1990). Introduction to Plant Disease Epidemiology. New York, EEUU: John Wiley and Sons.
Cirulli, M., Colella, C., D’Amico, M., Amenduni, M. y Bubici, G. (2008). Comparison of screening methods for the evaluation of olive resistente to Verticillium dahliae Kleb. Journal of Plant Pathology, 90, 7-14.
Cotton Research and Development Corporation (2017). Breaking the Verticillium cycle. En: Cotton Research and Development Corporation Annual Report. (N° 2016-2017). Narrabri, Australia: Cotton Research and Development Corporation. Recuperado de: http://www.insidecotton.com/xmlui bitstream/handle/1/4515/CRDC%20Annual%20Report%202016-17%20
-bookmarks.pdf?sequence=3&isAllowed=y
Dadd Daigle, P., Kirkby, K., Collins, D., Cuddy, W., Lonergan, P., Roser, S., …y Chapman T. (2020). Virulence not linked with vegetative compatibility groups in Australian cotton Verticillium dahliae isolates. Australian Journal of Crop Science, 14, (4), 633-640. DOI: https://doi.org/10.21475/ajcs.20.14.04.p2208
Dervis, S., Mercado Blanco, J., Erten L., Valverde Corredor, A. y Pérez Artés, E. (2010). Verticillium wilt of olive in Turkey: a survey on disease importance, pathogen diversity and susceptibility of relevant olive cultivars. European Journal of Plant Pathology, 127,
287–301. DOI: https://doi.org/10.1007/s10658-010-9595-zç
Devey, M. y Rosielle, A. (1986) Relationship between field and greenhouse ratings for tolerance to Verticillium wilt on cotton. Crop Science, 26, 1–4. DOI: https://doi.org/10.2135/cropsci1986.0011183X002600010001x
Docampo, D. M., Vázquez, A. A. y Laguna, I. G. (1981). Verticillium dahliae Kleb causal de la parálisis parcial del olivo en el área olivera centro oeste Argentina. Revista de Investigaciones Agropecuarias, 16 (2), 197-204.
Doyle, J. J. y Doyle, J. L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12, 13-15.
Gómez del Campo, M., Morales Sillero, A., Vita Serman, F., Rousseaux, M. C. y Searles P. S. (2010). El Olivar en los Valles áridos del Noroeste de Argentina (provincias de Catamarca, La Rioja y San Juan). Olivae, 114,23-45. Recuperado de: http://hdl.handle.net/11336/68724
Di Rienzo, J. A., Casanoves, F., Balzarini, M. G., Gonzalez, L., Tablada, M. y Robledo, C. W. Infostat (versión 2019) [Software] Córdoba, Argentina: Grupo InfoStat,
Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba. URL: http://www.infostat.com.ar
Jiménez Díaz, R. M., Cirulli, M., Bubici, G., Jiménez Gasco, M. M., Antoniou, P. y Tjamos, E. C. (2012). Verticillium wilt: a major threat to olive production. Current status and future prospects for its management. Plant Disease, 96 (3), 304-329. DOI: https://doi.org/10.1094/PDIS-06-11-0496
Jiménez Díaz, R. M., Olivares García, C., Trapero Casas, J. L., Jiménez Gasco, M. M., Navas Cortés, J. A., Landa, B. B., Milgroom, M. G. (2017). Variation of pathotypes
and races and their correlations with clonal lineages in Verticillium dahliae. Plant Pathology, 66 (4), 651-666. DOI: https://doi.org/10.1111/ppa.12611
Kheiri, A. y Fatahi, M. (2010). Evaluation of Verticillium Wilt Tolerance in Different Cotton Cultivars. Journal of Research in Agricultural Science, 6, 55-61.
Ladux, J. L., Jotayan, L., Otero, M. L., González Vera, C. y Ortiz J. (2014). Incidence of Verticillium dahlia in traditional orchards of the olive “Arauco” in northwest Argentina (La Rioja). Acta Horticulturae, 1057, 127-131. DOI: https://doi.org/10.17660/ActaHortic.2014.1057.14
Lachquer, K. y Sedra M. H. (2002). Characterisation of Verticillium dahliae Kleb. isolates from Olea europaea using RAPD markers. Phytopathologia Mediterranea,
41(3), 170–178.https://oajournals.fupress.net/index.php/pm/article/view/4974
López Escudero, J., Nuñez Santos, D. y Blanco López, M. (2003). Aislamiento de Verticillium dahliae de suelo y caracterización morfológica de sus microesclerocios.
Boletín de Sanidad Vegetal. Plagas, 29, 613-626.
Melouk, H. (1992). Verticillium. En L. L. Singleton, J. D. Mihail, y C. M. Rush (Eds.), Methods for research on soilborne pathogenic fungi (175–178). Saint Paul, Minnesota, EE. UU.: American Phytopathological Society Press.
Mercado Blanco, J., Rodríguez Jurado, D., Pérez Artes, E. y Jiménez Díaz, R. M. (2001). Detection of the non-defoliating pathotype of Verticillium dahliae in infected olive plants by nested PCR. Plant Pathology, 50 (5), 609-619.DOI: https://doi.org/10.1046/j.1365-3059.2001.00601.x
Mercado Blanco, J., Rodríguez Jurado, D., Pérez Artes, E. y Jiménez Díaz, R. M. (2002). Detection of the defoliating pathotype of Verticillium dahliae in infected olive plants by nested PCR. European Journal of Plant Pathology, 108, 1–13. DOI: https://doi.org/10.1023/A:1013994827836
Mercado Blanco, J., Rodríguez Jurado, D., Parrilla Araujo, S. y Jiménez Díaz, R. M. (2003). Simultaneous detection of the defoliating and nondefoliating Verticillium dahliae pathotypes in infected olive plants by duplex, nested polymerase chain reaction. Plant Disease, 87 (12),1487-1494.DOI: https://doi.org/10.1094/PDIS.2003.87.12.1487
Morera, B., Páez, J. I., Vega, J. M. y Montes, F. (2005). Comparación de métodos de diagnóstico de Verticillium dahliae en olivo: aislamiento en medio de cultivo y PCR. Boletín de Sanidad Vegetal. Plagas, 31, 267-275.
Nazar, R. N., Hu, X., Schmidt, J., Culham, D. y Rob, J. (1991). Potencial use of PCR-amplified ribosomal intergenic sequences in the detection and differentiation of Verticillium wilt pathogens. Physiological and Molecular Plant Pathology, 39, 1-11.
DOI: https://doi.org/10.1016/0885-5765(91)90027-F
Otero, M. L. (2005). Caracterización biológica, serológica y molecular de Verticillium dahliae en olivos de la provincia de La Rioja. (Tesis doctoral no publicada). Facultad de Ciencias Exactas, Universidad Nacional de Córdoba. Córdoba, Argentina.
Pegg, G. F. y Brady, B. L. (2002). Verticillium wilts. Wallingford, EEUU: CABI Publishing.
Pérez Artes, E., García Pedrajas, M. D., Béjarano Alcázar, J. y Jiménez Díaz, R. (2000). Differentiation of cotton-deffoliating and nondefoliating pathotypes of Verticillium dahliae by RAPD and specific PCR analyses. European Journal Plant Pathology, 106, 507-517.
Presley, J. T. (1969). Growth response of Verticillium alboatrum to sanguinarine in nutrient agar. Phytopathology, 59,1968-1969.
Roca, M., Docampo, D., Otero, M., Babbitt, S., Barreto, D., Costilla, M., ... y Guchea, F. (2001). La rama seca en olivares de la Provincia de La Rioja. Revista Sociedad
Argentina de Horticultura, 20, 58.
Roca, M. (2015). Revisión de antecedentes, presencia, incidencia, severidad, frecuencia de agentes asociados y mermas de rendimiento para “rama seca” del olivo en la provincia de La Rioja. (Tesis de maestría). Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, La Plata, Argentina. DOI: https://doi.org/10.35537/10915/59879
Rodríguez Jurado, D. (1993). Interacciones huéspedparásito en la Verticilosis del olivo (Olea europaea L.) inducida por Verticillium dahliae Kleb. (Tesis doctoral no publicada). Universidad de Córdoba, Córdoba, España.
Schnathorst, W. C., y Sibbet, G. S. (1971). The relation of strains of Verticillium alboatrum to severity of Vertillium wilt in Gossypium hirsutum and Olea europaea in
California. Plant Disease Reporter, 9, 780-782.
Zhou, H., Fang, H., Sanogo, S., Hughs, S. E., Jones D. C., and Zhang, J. (2014). Evaluation of Verticillium wilt resistance in commercial cultivars and advanced
breeding lines of cotton. Euphytica, 196, 437-448. DOI: https://doi.org/10.1007/s10681-013-1045-5