Characterization of grapevine (Vitis vinifera L.) varieties and rootstocks by root isoenzymes
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Abstract
Twenty-one cultivars of Vitis vinifera L. and three rootstocks were chosen to study their biochemical characterization based on the electrophoretic separation of isoenzyme systems obtained from root tissue extracts. Acid phosphatases (ACPH), esterases (EST), catechol oxidases (CO), glutamate oxaloacetate transaminases (GOT), malate dehydrogenases (MDH), and peroxidases (PER) were separated using polyacrylamide gels and later stained with specific stains for the various isoenzyme systems. ACPH, CO, EST, and MDH were found to be the most adequate systems for characterization. The results of the study of the different systems and the grouping of the cultivars are presented. The use of isoenzymatic techniques for the characterization of Vitis genotypes is suggested.
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