Use of Bacillus velezensis RI3 and Pseudomonas psychrophila P10 strains as plant growth-promoting rhizobacteria in lettuce (Lactuca sativa L.)
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Lettuce is cultivated on over one million hectares worldwide, with Argentina contributing 40,000 ha. Córdoba province is the second-largest producer in Argentina, but its green belt has seen significant reductions in cultivated areas. New environmentally friendly technologies are needed to improve, sustain and increase lettuce volume production. This study aimed to use plant growth-promoting rhizobacteria (PGPR) as a sustainable alternative to enhance lettuce production (Lactuca sativa L.) growth while mitigating environmental impact. Two PGPR strains, Bacillus velezensis RI3 and Pseudomonas psychrophila P10, were tested in both in vitro and field experiments. Parameters such as shoot dry weight, root dry weight, evapotranspiration, and water use efficiency were assessed. The in vitro study results demonstrated significant improvements in shoot and root dry weights for both inoculated treatments compared to the control. Field experiments, conducted on 90 m² plots, also demonstrated significant increases in shoot dry weight, with Pseudomonas psychrophila showing a 28.57 % increase and Bacillus velezensis a 8.44 % increase compared to controls. This study highlights the potential of Bacillus velezensis and Pseudomonas psychrophila as effective biofertilizers, promoting sustainable lettuce cultivation. Further research is recommended to explore the mechanisms of plant-microbe interactions to optimize PGPR application in agricultural practices.
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